Composite

Part:BBa_K311001:Design

Designed by: Matthew Adams, Poonam Srivastava, Ian Windsor   Group: iGEM10_Minnesota   (2010-10-15)


Strong and constitutive tet promoter with downstream mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the promoter with mRFP was obtained by digesting the plasmid J61002 with EcoRI and Pst I and ligating it to Eco RI and Pst I cut pSB1C3.


Source

It came from the part J23118 and plasmid J61002.

References